Open AccessCloning of genes encoding coli‐surface (CS) antigens in enterotoxigenic Escherichia coli
Author(s) -
Willshaw Geraldine A.,
Smith Henry R.,
McConnell Moyra M.,
Rowe Bernard
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02779.x
Subject(s) - plasmid , escherichia coli , recombinant dna , biology , antigen , enterotoxigenic escherichia coli , cloning (programming) , microbiology and biotechnology , gene , colonisation , molecular cloning , genetics , gene expression , colonization , enterotoxin , computer science , programming language
Sequences encoding the CS6 antigen of colonisation factor antigen (CFA)IV were cloned on a 3kb Cla I fragment. The recombinant plasmid pDEP5 coded for surface expression of CS6 measured by ELISA and production of CS6 polypeptides was detected in E. coli minicells. The genes for the CS1, CS2 and CS3 components of colonisation factor antigen CFA/II were cloned together on a length of DNA corresponding to about 17kb. CS3 was always expressed but production of either CS1 or CS2 depended on the serotype and biotype of the host strain. Separate subclones were obtained that expressed CS3 or CS1 and CS2.