Open Accessα‐glucosidase in Mycoplasma mycoides subspecies capri
Author(s) -
Wadher B.J.,
Miles R.J.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02776.x
Subject(s) - mycoplasma mycoides , maltose , microbiology and biotechnology , biology , biochemistry , mycoplasma , extracellular , enzyme , chemistry
Mycoplasma mycoides subsp. capri utilisede maltose in medium lacking serum and hence serum saccharolytic enzymes. The presence of α‐glucosidase activity was demonstrated by p‐nitrophenyl‐α‐ d ‐glucoside hydrolysis in toluene‐treated cells. Specific activities were approx. 4‐fold higher in cells grown in the presence of maltose than in cells grown with other sugars or with glucose plus maltose. Extracellular activity was < 2% of cellular activity in growing cultures. α‐Glucosidase activity was also demonstrated in cells grown in medium containing serum. It is suggested that the presence of α‐glucosidase might be of value in mycoplasma chatacterisation; in a previous study, α‐glucosidase activity was not detected in Mycoplasma mycoides subsp. mycoides .