Open AccessInsertional inactivation by Tn3701 of pIP964 hemolysin expression in Enterococcus faecalis
Author(s) -
Le Bouguénec Chantal,
Horaud Théa,
Geoffroy Christiane,
Alouf J.E.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02775.x
Subject(s) - hemolysin , hemolysis , complementation , plasmid , ecori , microbiology and biotechnology , enterococcus faecalis , biology , enterococcus , gene , genetics , virulence , escherichia coli , phenotype , immunology , antibiotics
Transposition of the conjugative mobile element (Tn 3701 ) of Etreptococcus pyogenes strain A454 onto the Enterococcus feacalis hemolysin plasmid pIP964 modified the expression of the genes involved in hemolysin production. By hemolysis complementation tests the genes coding for two components required for the hemolytic activity expression were found to be located on the Eco RI E and G fragments of pIP964.