Open AccessStudy of a fructose‐negative mutant of Rhizobium meliloti
Author(s) -
El Guezzar Mounir,
Hornez JeanPierre,
Courtois Bernard,
Derieux JeanClaude
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02770.x
Subject(s) - fructokinase , fructose , mutant , rhizobium , glucose 6 phosphate isomerase , biochemistry , mutagenesis , aldolase a , biology , strain (injury) , enzyme , fructose 1,6 bisphosphatase , fructolysis , aldolase b , fructose bisphosphate aldolase , isomerase , sinorhizobium meliloti , gene , anatomy
Rhizobium meliloti grows on fructose as sole carbon source. Following nitrosoguanidine mutagenesis, a mutant of R. meliloti M5N1 was isolated as unable to grow on fructose. Enzyme assays with cell‐free extracts showed it to lack significative phosphoglucose isomerase activity. Other enzymes were present at low levels. Both fructose and fructose 6‐phosphate were accumulated within this mutant. The in vitro inhibition of fructokinase by fructose 6‐phosphate was show. Symbiotic properties remained unaffected in the mutant strain.