Open AccessCharacterization of two Bacillus thuringiensis plasmids whose replication is thermosensitive in B. subtilis
Author(s) -
Lereclus D.,
Guo S.,
Sanchis V.,
Lecadet M.M.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02768.x
Subject(s) - plasmid , bacillus thuringiensis , transposable element , bacillus subtilis , biology , escherichia coli , bacillaceae , genetics , restriction map , rolling circle replication , dna , microbiology and biotechnology , dna replication , gene , bacteria , genome
Two cryptic plasmids of 8.6 and 15 kb, originating from Bacillus thuringiensis , have been cloned in Escherichia coli . The determination of their physical map shows that the 8.6‐kb plasmid harbors the transposon Tn 4430 and that the 15‐kb plasmid carries Tn 4430 plus one copy of the IS 231 element. The replication regions were identified on the restriction maps and the segregational stability of derived plasmids containing these regions was analyzed in B. subtillis . The results indicate that the stability of these plasmids is negatively correlated to the temperature. After 30 generations, without selective pressure at 51°C, the two types of plasmids are lost.