Open AccessExpression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis
Author(s) -
Lejeune André,
Eveleigh Douglas E.,
Colson Charles
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02758.x
Subject(s) - zymomonas mobilis , pseudomonas fluorescens , biology , pseudomonas , expression vector , plasmid , gene , pseudomonadales , pseudomonadaceae , microbiology and biotechnology , bacteria , biochemistry , genetics , recombinant dna , fermentation , ethanol fuel
Using a broad host‐range, mobilizable plasmid vector, the endoglucanase gene eglX from Pseudomonas fluorescens var. cellulosa was cloned and expressed in the bacterial ethanologen Zymomonas mobilis . The enzyme was intracellular in this new host. It was produced throughout the growth phase and was not repressed by glucose. We postulate that transcription of the eglX gene was initiated from a promoter of the vector in Z. mobilis .