Open AccessCloning and expression of the 3‐isopropylmalate dehydrogenase gene from Candida albicans
Author(s) -
Jenkinson Howard F.,
Schep Gillian P.,
Shepherd Maxwell G.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02731.x
Subject(s) - complementation , gene , biology , candida albicans , ura3 , transformation (genetics) , cloning (programming) , genetics , saccharomyces cerevisiae , molecular cloning , escherichia coli , microbiology and biotechnology , gene expression , phenotype , computer science , programming language
A variety of Saccharomyces cerevisiae genes e.g. HIS3, LEU2, TRP1, URA3 , are expressed in Escherichia coli and have been isolated by complementation of mutations in the corresponding E. coli genes [1]. The LEU2 gene was one of the first S. cerevisiae genes to be isolated in this way [2], and its isolation led to the development of transformation systems for S. cerevisiae [3,4]. The leuB gene in E. coli [5] and the LEU2 gene in S. cerevisiae [6] both code for 3‐isopropylmalate dehydrogenase (3‐IMDH; EC 1.1.1.85) which is essential for the biosynthesis of leucine in both organisms. This paper describes the cloning of a fragment of C. albicans DNA carrying the gene for 3‐IMDH which will be useful in the development of transformation methods in C. albicans .