Open AccessRole of IncP‐1 plasmid primase in conjugation between Pseudomonas species
Author(s) -
Nash J.,
Krishnapillai V.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02726.x
Subject(s) - primase , plasmid , pseudomonas stutzeri , biology , dna , dnag , genetics , microbiology and biotechnology , gene , circular bacterial chromosome , bacteria , dna replication , polymerase chain reaction , reverse transcriptase
A Tn7 insertion in the DNA primase gene of the promiscuous IncP‐1 plasmid R18 specifically reduced plasmid conjugational transfer from Pseudomanas aeruginosa donors to Pseudomonas stutzeri recipients. The cloned primase gene was found to efficiently complement the mutation in both the donor and in the recipient suggesting that the primase is required for priming single‐stranded plasmid DNA in the donor prior to its transit to the recipient where it is converted to the double‐ stranded form.