Open AccessInitiation of glucan synthesis in yeast
Author(s) -
Andaluz E.,
Ridruejo J.C.,
Ramírez M.,
RuízHerrera J.,
Larriba G.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02725.x
Subject(s) - yeast , candida albicans , glucan , papain , biochemistry , chemistry , gel electrophoresis , solubilization , chromatography , sodium , enzyme , biology , microbiology and biotechnology , organic chemistry
Fractions of the glucan synthesized in vitro by glucan synthase preparations obtained by mechanical breakage of whole cells of Sacchromyces cerevisiae and Candida albicans were solubilized by sodium dodecyl sulphate (SDS). Part of this material migrated in denaturing electrophoresis at the level of a Coomassie Blue‐stained area. In addition, it was solubilized by both an exoglucanase and papain. These results suggest that some yeast glucan is built, associated with a protein.