Open AccessRapid determination of Microcystis sp. toxins by reversed‐phase liquid chromatography
Author(s) -
Dierstein Roland,
Kaiser Ines,
Weckesser Jürgen
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02705.x
Subject(s) - chromatography , bioassay , microcystis , elution , chemistry , cartridge , high performance liquid chromatography , solid phase extraction , extraction (chemistry) , peptide , biology , cyanobacteria , bacteria , biochemistry , materials science , genetics , metallurgy
A rapid procedure is described for the identification of peptide toxins from Microcystis sp. strains. The amphiphilic toxins were isolated by (1) water extraction of dried cells, (2) adsorption onto reversed‐phase C 18 cartridges and differential elution, and (3) high‐performance liquid chromatography. The method is suitable for quantification and might substitute for the mouse bioassay.