Open AccessCloning and expression of the Staphylococcus aureus glucosaminidase in Escherichia coli
Author(s) -
Biavasco F.,
Pruzzo C.,
Thomas C.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02696.x
Subject(s) - escherichia coli , staphylococcus aureus , microbiology and biotechnology , cloning (programming) , biology , molecular cloning , enterobacteriaceae , bacteria , gene , gene expression , genetics , computer science , programming language
A fragment of Staphylococcus aureus DNA encoding the glucosaminidase determinant was cloned in Escherichia coli by inserting the Sau 3A genomic fragments in the Bam HI site of the plasmid vector pBR322. One clone selected on the basis of its lytic activity was shown to contain a hybrid plasmid (pEU213) carrying a 4.7 kb insert of S. aureus DNA. Lytic activity was tested using different assays, and the enzyme production was confirmed by immunological reactions. An appreciable reduction of lytic activity was noted after few subcultures. The E. coli carrying pEU213 had a slower growth rate and increased autolytic activity compared to the parental strain. The possible reasons for this behavior are discussed.