Open AccessDifferent effects of 5‐fluorouracil on Methanosarcina barkeri and on Methanobacterium thermoautotrophicum
Author(s) -
Grinbergs Andrejs,
Müller Volker,
Gottschalk Gerhard,
Thauer Rudolf K.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02679.x
Subject(s) - methanosarcina barkeri , methanobacterium , uracil , strain (injury) , biology , dna synthesis , bacteria , biochemistry , nucleic acid , stereochemistry , dna , microbiology and biotechnology , chemistry , archaea , genetics , methanogenesis , anatomy
Abstract Growth of Methanosarcina barkeri (strain Fusaro) was found to be inhibited by 5‐fluorouracil (FU) only at relatively high concentrations (>50 μg / ml ). Inhibition could not be relieved by uracil. Therefore, FU probably did not exert its effect via inhibition of DNA synthesis as is the case in other organisms. Control experiments with Methanobacterium thermoautotrophicum (strain Marburg) on the other hand revealed that the effect of FU on this archaebacterium is probably exerted at the level of nucleic acid synthesis. The M. thermoautotrophicum cultures rapidly acquired resistance towards the pyramidine analog.