Open AccessIsolation and characterization of methanogenic bacteria from rice paddies
Author(s) -
Rajagopal B.S.,
Belay Negash,
Daniels Lacy
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02659.x
Subject(s) - methanosarcina , methanobacterium , methanogenesis , sulfur , bacteria , methanol , methanosarcina barkeri , methylamines , methanogen , energy source , chemistry , biology , food science , enrichment culture , archaea , biochemistry , organic chemistry , catalysis , coal , genetics
Enrichment cultures for H 2 ‐CO 2 , methanol‐ or acetate‐utilizing methanogens were prepared from two rice field soil samples. All the cultures except one acetate enrichment showed significant methane production. Pure cultures of Methanobacterium ‐ and Methanosarcina ‐like organisms were isolated from H 2 ‐CO 2 and methanol enrichment cultures, respectively, and were characterized for various nutritional and growth conditions. The organisms had an optimal pH range of 6.4–6.6 and a temperature optimum of 37°C. The Methanobacterium isolates were able to utilize H 2 ‐CO 2 but no other substrates as sole energy source, while the Methanosarcina isolates were able to utilize methanol, methylamines or H 2 ‐CO 2 as sole energy sources. Both Methanobacterium isolates and one isolate of Methanosarcina were able to use dinitrogen as the sole source of nitrogen for growth. The isolates used several sulfur compounds as sole sources of sulfur.