Open AccessMicroaerophilic growth of Wolinella recta ATCC 33238
Author(s) -
Ohta Hiroyuki,
Gottschal Jan C.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02650.x
Subject(s) - chemostat , formate , microaerophile , electron acceptor , oxygen , aeration , chemistry , biochemistry , respiration , biology , nuclear chemistry , bacteria , organic chemistry , botany , genetics , catalysis
The influence of oxygen on growth and fumarate‐dependent respiration of Wolinella recta ATCC 33238 was studied in continuous culture. Steady states were obtained with formate‐limited cultures grown at a specific growth rate of 0.1 h −1 with different levels of oxygenation. The extent of aeration was regulated by means of a redox control system permitting reproducible cultivation at oxygen levels below the detection limit of conventional lead‐silver probes. The ratio of succinate produced to that of formate consumed (Suc/For) decreased from 0.99 in strictly anaerobic cultures to 0.06–0.10 in aerated cultures. The growth yield did not change significantly with increasing redox readings: 4.9–5.2 g cell carbon/mol formate. The ability to use O 2 as the sole electron acceptor was demonstrated in a chemostat culture with formate as electron donor and succinate as carbon source. Washed cells from all chemostat cultures comsumed O 2 with formate as electron donor at a high rate (2.1–3.7 μmol/min per mg protein) and possessed b ‐ and c ‐type cytochromes and CO‐binding pigments. These results clearly indicated the microaerophilic nature of W. recta .