Open AccessExpression of heterologous genes in Pseudomonas putida under the control of streptococcal promoters
Author(s) -
Laplace Frank,
Müller Jörg,
Wollweber Leo,
Malke Horst
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02610.x
Subject(s) - pseudomonas putida , periplasmic space , heterologous , promoter , heterologous expression , biology , plasmid , pseudomonas , recombinant dna , microbiology and biotechnology , gene , genetics , gene expression , bacteria , escherichia coli
Streptococcal promoters were shown to fulfil primary structure requirements for the expression of heterologous genes in Pseudomonas putida KT2440. The identity of the gene products, streptokinase and human interferon‐α1, as synthesized by KT2440, was confirmed by their biological, antigenic and/or physical properties. Although designed as secretion vectors with streptococcal signal sequences, the recombinant plasmids failed to mediate the complete export of either protein into the periplasmic space of KT2440.