Open AccessGenome fingerprinting of Pseudomonas aeruginosa by two‐dimensional field inversion gel electrophoresis
Author(s) -
Bautsch Wilfried,
Grothues Dietmar,
Tümmler Burkhard
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02606.x
Subject(s) - genome , restriction enzyme , biology , pseudomonas aeruginosa , typing , restriction fragment , genetics , ecori , restriction fragment length polymorphism , gel electrophoresis , dna profiling , terminal restriction fragment length polymorphism , restriction map , microbiology and biotechnology , bacteria , gene , dna , genotype , base sequence
Genome analysis of Pseudomonas aeruginosa was performed by digestion with rare‐cutting restriction endonucleases and subsequent one‐ and two‐dimensional field inversion gel electrophoresis (FIGE). The frequency of chromosomal recognition sites increased in the order Spe I, Dra I, Xba I, Ssp I, Nhe I. The genome size of strain PAO and the 17 IATS strains varied from 4.4 × 10 6 to 5.4 × 10 6 base pairs. Double restriction digests and two‐dimensional FIGE provide a genome fingerprint which is useful for the identification and typing of the respective strains.