Open AccessStructural rearrangements of a broad host range plasmid encoding mercury resistance from an epilithic isolate of Pseudomonas cepacia
Author(s) -
Rochelle P.A.,
Day M.J.,
Fry J.C.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02604.x
Subject(s) - plasmid , pseudomonas aeruginosa , microbiology and biotechnology , biology , pseudomonas fluorescens , pseudomonadaceae , pseudomonas , pseudomonadales , dna , bacteria , genetics
A broad host range plasmid encoding mercury resistance (pQM3) underwent structural rearrangement when transferred into Pseudomonas aeruginosa . The rearrangement involved deletions and additions of DNA to the plasmid and resulted in the formation of smaller derivative plasmids. A 13 kb insert acquired in an epilithic Pseudomonas fluorescens isolate stabilised pQM3 and prevented rearrangements in P. aeruginosa . Integration of pQM3 into the chromosome of its natural host Pseudomonas cepacia PAR 161, and the P. aeruginosa chromosome reduced its transfer efficiency.