Open AccessIdentification of two new denitrifying strains of Rhodobacter sphaeroides
Author(s) -
Michalski Wojciech P.,
Nicholas D.J.D.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02603.x
Subject(s) - rhodobacter sphaeroides , denitrifying bacteria , nitrite reductase , nitrate reductase , denitrification , rhodospirillaceae , nitrite , nitrate , rhodospirillales , microbiology and biotechnology , chemistry , rhodobacter , biochemistry , plasmid , biology , bacteria , enzyme , photosynthesis , gene , genetics , organic chemistry , nitrogen , mutant
Highly specific polyclonal and antibodies against either nitrate, nitrite or nitrous oxide reductases from a photosynthetic denitrifying bacterium Rhodobacter sphaeroides f. sp. denitrificans were used to show the presence of immunologically reactive proteins in strains that Pellerin and Gest had shown to grow in the dark with nitrate as a terminal acceptor [9]. Two strains of this bacterium, namely 81‐3 and 2.4.3 synthesized the three denitrifying enzymes and were capable of denitrification. Strains 81‐1 and 2.4.1 (neotype) both expressed nitrate reductase activities but nitrite reductase was not detected since these strains did not reduce nitrite. They also did not grow in the dark with nitrate as a terminal acceptor. Each of strains 81‐1, 81‐3, 2.4.1 and 2.4.3 contain four plasmids. R. sphaeroides f. sp. denitrificans , however, contains only one large 108 kb plasmid, which is distinctly different in size from those detected in the other strains. This indicates that the 108 kb plasmid is not necessarily specific for denitrification.