Open AccessMvn I: A restriction enzyme in the archaebacterium Methanococcus vannielii
Author(s) -
Thomm Michael,
Frey Gerhard,
Bolton Bryan J.,
Laue Frank,
Kessler Christoph,
Stetter Karl O.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02601.x
Subject(s) - methanococcus , restriction enzyme , enzyme , biochemistry , biology , isoschizomer , chemistry , dna , stereochemistry , archaea , gene , gene expression , dna methylation
The methanogenic archaebacerium Methanococcus vannielii contains a type II restriction endonuclease. The enzyme was purified by a simple three‐step procedure resulting in enzyme preparations free of contaminating unspecific nucleases. The restriction enzyme recognizes and cleaves the sequence 5′‐CG ↓ CG‐3′ ( Fnu DII and Tha I isoschizomer) and generates DNA fragments with blunt ends. Due to its purity and activity at moderate temperatures, Mvn I might be a useful alternative to Fnu DII and Tha I active at 60°C.