Open AccessA system for the inducible secretion of proteins from Bacillus subtilis during logarithmic growth
Author(s) -
Edelman Alex,
Joliff Gwennaël,
Klier André,
Rapoport Georges
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02581.x
Subject(s) - levansucrase , bacillus subtilis , shuttle vector , signal peptide , escherichia coli , biology , gene , expression vector , bacillaceae , nucleic acid sequence , microbiology and biotechnology , recombinant dna , biochemistry , genetics , vector (molecular biology) , bacteria
A Bacillus subtilis‐Escherichia coli shuttle vector was constructed containing the B. subtilis levansucrase gene promoter and region encoding its signal sequence. A site for the restriction enzyme Nae I was included to facilitate precise translational fusions to the DNA encoding the levansucrase signal sequence. Fusions of TEM β‐lactamase to this construct displayed sucrose‐inducible expression and secretion of B. subtilis .