Open AccessPurification and characterization of heat‐labile enterotoxin isolated from chicken enterotoxigenic Escherichia coli
Author(s) -
Tsuji Takao,
Encarnacion Joya Josephine,
Yao Shukun,
Honda Takeshi,
Miwatani Toshio
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02575.x
Subject(s) - enterotoxigenic escherichia coli , enterotoxin , escherichia coli , heat stable enterotoxin , heat labile enterotoxin , biology , ouchterlony double immunodiffusion , gel electrophoresis , protein subunit , microbiology and biotechnology , polyacrylamide gel electrophoresis , antigen , chemistry , biochemistry , enzyme , antiserum , gene , immunology
The heat‐labile enterotoxin (LTc) isolated from chicken enterotoxigenic Escherichia coli was purified to homogeneity and its molecular and antigenic properties were compared with those of purified LTs from porcine and human enterotoxigenic Escherichia coli (LTp, LTh). The A subunit of LTc was identical to that of LTp and the B subunit of LTc was identical to that of LTh but not that of LTp, in mobility on SDS‐polyacrylamide gel electrophoresis. Ouchterlony tests demonstrated that LTc is antigenically identical to LTh but not with LTp. The p I point and amino acid composition of LTc were also compared and the results suggest that chicken enterotoxigenic E. coli produced an LT similar to LTh.