Open AccessAssessment of the toxicity of the cloned 27 kDa toxin of Bacillus thuringiensis var. morrisoni strain PG14
Author(s) -
Earp David J.,
Ellar David J.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02567.x
Subject(s) - toxin , bacillus subtilis , bacillus thuringiensis , microbiology and biotechnology , biology , toxicity , in vitro , strain (injury) , in vivo , bacillales , bacteria , bacillaceae , biochemistry , chemistry , genetics , organic chemistry , anatomy
Expression of the cloned 27 kDa toxin gene from Bacillus thuringiensis strain PG14 in Bacillus subtilis facilitated the evaluation of the in vivo and in vitro toxicities of the pure toxin. This toxin is known to differ from the 27 kDa δ‐endotoxin of var. israelensis by a single amino acid. Comparison of toxicity values with data obtained from similarly produced 27 kDa toxin from var. israelensis suggested that the amino acid difference has no detectable effect on the potency of the toxin under the assay conditions employed.