Open AccessA rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay
Author(s) -
Matsumura K.,
Wakatsuki S.,
Iwasaki A.,
Endo R.,
Tanaka K.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02495.x
Subject(s) - immunoassay , hbsag , nitrocellulose , antiserum , chromatography , chemistry , antigen , microbiology and biotechnology , antibody , virology , biology , immunology , biochemistry , hepatitis b virus , membrane , virus
Abstract This study describes a dot enzyme immunoassay (Dot‐EIA) for detecting hepatitis B surface antigen (HBsAg). The results demonstrated that the detection level of this assay for HBsAg was 1.5 ng/ml; no false‐positive or ‐negative readings were observed. Also, this Dot‐EIA had some advantages over standard EIA: (1) antiserum could be directly and immediately bound on nitrocellulose paper set into microfiltration apparatus, (2) the paper could be easily washed under reduced pressure using a water aspirator, (3) all assay steps could be performed at room temperature within 2 h, (4) the well‐defined brown spots could be evaluated by both visual observation and densitometric reading. The Dot‐EIA reported here may be useful for rapid diagnosis and screening of HBsAg in serum.