Open AccessA sensitive chemiluminescence assay for pertussis toxin and for evaluation of cell‐free pertussis toxoids
Author(s) -
Craig F.F.,
Christodoulides M.,
Parton R.,
StewartTull D.E.S.,
Freer J.H.,
Lackie J.M.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02494.x
Subject(s) - pertussis toxin , chemiluminescence , bordetella pertussis , toxin , chemistry , microbiology and biotechnology , luminol , bordetella , carbodiimide , biochemistry , chromatography , biology , bacteria , receptor , g protein , genetics
Pertussis toxin (PT) inhibited luminol‐enhanced chemiluminescence induced in rabbit peritoneal neutrophils by N′‐formyl‐ l ‐methionyl‐ l ‐leucyl‐ l ‐phenylalanine (fMLP) at doses as low as 0.8 ng·ml −1 , even in the presence of a 10‐fold higher concentration of filamentous haemagglutinin (FHA). A cell‐free extract of Bordetella pertusis , containing predominantly PT and FHA, suppressed the neutrophil response to fMLP. After toxoiding with carbodiimide, the inhibitory activity of the extract was abolished and an enhancement of neutrophil chemiluminescence was observed due to FHA activity. Abrogation of the chemiluminescent response of neutrophils to fMLP is proposed as a sensitive, in vitro assay for PT, and may be useful for monitoring the residual toxin activity in pertussis toxoids and for determining the anti‐toxic effects of anti‐PT antibodies.