
Unusual cross‐reactions among monoclonal antibodies to bacterial antigens: idiotypic and competitive binding analysis
Author(s) -
Ghosh Souravi,
Campbell Ailsa M.
Publication year - 1988
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1988.tb02484.x
Subject(s) - monoclonal antibody , antiserum , antigen , serotype , antibody , competitive binding , biology , vibrio cholerae , microbiology and biotechnology , virology , bacteria , biochemistry , immunology , receptor , genetics
In a previous study, we have described unusual cross‐reactions among monoclonal antibodies (Mabs) to bacteria and in particular to the Inaba and Ogawa serotypes of Vibrio cholerae . In this study, the extent to which the binding sites of both antibodies and antigens overlap has been investigated by competitive binding and idiotypic analysis. The competitive binding data indicate that the cross‐reactive binding of the Inaba Mabs to the Ogawa vibrios can be abolished by incubation with higher affinity Ogawa Mabs. However, rabbit antiserum raised against the Inaba series does not react with the Ogawa series, indicating that anti‐Inaba Mabs do not share idiotypic determinants with anti‐Ogawa Mabs. The results therefore suggest that the two sets of antibodies recognise different determinants which are closely related in spatial terms, and which consequently do not permit simultaneous binding of the two types of monoclonal antibody.