Open AccessA simple method for purification of Shiga or Shiga‐like toxin from Shigella dysenteriae and Escherichia coli O157: H7 by immunoaffinity column chromatography
Author(s) -
Kongmuang Urirat,
Honda Takeshi,
Miwatani Toshio
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02628.x
Subject(s) - shigella dysenteriae , shiga toxin , escherichia coli , toxin , affinity chromatography , biology , microbiology and biotechnology , shigella , chromatography , shiga like toxin , escherichia , column chromatography , chemistry , biochemistry , enzyme , gene
A simple method was developed for purifying Shiga toxin and Shiga‐like toxin from the culture supernatants and cell lysates of Shigella dysenteriae type 1 and Escherichia coli O157 : H7 grown in modified syncase media. Two steps, DEAE cellulose column chromatography and immunoaffinity column chromatography, were sufficient for obtaining purified toxin. By this procedure, about 0.32–0.75 mg of purified toxin was obtained from 5 1 of culture with high recovery rate (53–62%). The toxins purified by this method from the culture supernatants and cell lysates of S. dysenteriae and E. coli O15 : H7 were immunologically, biologically and structurally indistinguishable.