Open AccessWhole‐cell ELISA for typing Neisseria meningitidis with monoclonal antibodies
Author(s) -
Abdillahi Hussein,
Poolman Jan T.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02626.x
Subject(s) - neisseria meningitidis , monoclonal antibody , typing , antigen , microbiology and biotechnology , neisseria , neisseriaceae , biology , conjugate , antibody , bacteria , virology , immunology , genetics , antibiotics , mathematical analysis , mathematics
Large‐scale screening of Neisseria meningitidis strains is necessary for epidemiological studies as well as for identifying immunologically important antigens. We have developed a new and simpler type of ELISA for this purpose. Whole bacteria from the strains being studied are coated onto PVC plates; the type and subtype are then determined by the binding of monoclonal antibodies with known specificities, detected with a protein A‐conjugate. This technique is rapid and easy but still sensitive and reproducible and is thus highly suitable for screening for antigens in a relatively native state on large numbers of strains.