Open AccessPenicillin‐binding proteins 4 and 5 of Haemophilus influenzae are involved in cell wall incorporation
Author(s) -
Mendelman Paul M.,
Chaffin Donald O.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02549.x
Subject(s) - penicillin binding proteins , haemophilus influenzae , ampicillin , escherichia coli , microbiology and biotechnology , cell wall , penicillin , bacterial cell structure , antibiotics , amp resistance , haemophilus , chemistry , cephalosporin , bacteria , pasteurellaceae , biochemistry , biology , genetics , gene
Permeabilized cells of Haemophilus influenzae incorporate wall precursors into murein material in an ampicillin‐sensitive reaction. In resistant transformants that contain the low antibiotic affinity penicillin‐binding proteins (PBPs) 4 and 5, the sensitivity of this incorporation reaction to ampicillin is proportionally lower, suggesting a catalytic role for these proteins in wall synthesis. We conclude that, analogous to the reaction in Escherichia coli , PBPs 4 and 5 of H. influenzae have transpeptidase activity.