Open AccessEffect of milk on surface properties of Staphylococcus aureus from bovine mastitis
Author(s) -
Mamo Wubshet,
Rozgonyi Ferenc,
Hjertén Stellan,
Wadström Torkel
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02541.x
Subject(s) - incubation , mastitis , chemistry , bovine milk , salt (chemistry) , chromatography , staphylococcus aureus , agar , capsule , incubation period , agar plate , bacteria , growth medium , surface charge , food science , microbiology and biotechnology , biochemistry , biology , botany , organic chemistry , genetics
The surface hydrophobicity of cells of Staphylococcus aureus strains isolated from bovine mastitis grown on conventional agar and broth media was drastically reduced after incubation with bovine milk. Strains grown in high carbohydrate‐high salt media yielded cells with reduced surface hydrophobicity compared to cells grown in conventional media, and adding bovine milk to minimal medium also yielded cells with reduced surface hydrophobicity, as determined by hydrophobic interaction chromatography and the salt aggregation test. Incubation of strains in milk and growth in a medium supplemented with bovine milk also significantly changed bacterial surface charge as determined by free‐zone electrophoresis. Strains with high or with decreased adsorptive and aggregating properties did not produce surface capsule or slime. Heat treatment (60° C or 80° C) of the bacterial suspensions did not significantly change their adsorptive and aggregating properties.