Open AccessThe influence of temperature stress on protein synthesis in the Rhodomicrobium vannielii (RM5) and in a rifampicin‐resistant mutant R82
Author(s) -
Scott N.W.,
Dow C.S.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02532.x
Subject(s) - mutant , rna polymerase , protein biosynthesis , biology , rna , protein subunit , biochemistry , polymerase , microbiology and biotechnology , cell , dna synthesis , cell growth , dna , gene
A rifampicin‐resistant mutant of Rhodomicrobium vannielii was isolated which was found to be temperature‐sensitive for swarmer‐cell production. Two‐dimensional PAGE analysis of proteins synthesised by the mutant indicated that the alterations in cell growth related to a reduced ability of the cell to synthesise the β‐subunits of the DNA‐dependent RNA polymerase. These observations point to an autogenous regulatory system controlling the synthesis of RNA polymerase subunits in R. vannielii . Temperature stress also influenced the synthesis of a number of other proteins; several were identified as either cell‐specific or as heat‐shock proteins.