Open AccessCrystalline formate dehydrogenase from Candida methanolica
Author(s) -
Izumi Yoshikazu,
Kanzaki Hiroshi,
Morita Shigeru,
Yamada Hideaki
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02530.x
Subject(s) - formate dehydrogenase , formate , yeast , polyethylene glycol , chemistry , enzyme , methanol , biochemistry , yield (engineering) , chromatography , organic chemistry , materials science , catalysis , metallurgy
Formate dehydrogenase (EC 1.2.1.2) was purified about 38‐fold with an overall yield of 76% from a methanol‐utilizing yeast, Candida methanolica (ATCC26175), in 4 steps and, by adding polyethylene glycol, the enzyme was crystallised for the first time. The final preparation appeared to be homogeneous by the criteria of polyacrylamide electrophoresis and analytical centrifugation. Compared with the yeast formate dehydrogenases so far reported, the purified enzyme exhibited higher specific activity (7.52 U/mg).