Open AccessTandem location of the cellulase genes on the chromosome of Bacillus sp. strain N‐4
Author(s) -
Fukumori Fumiyasu,
Ohishi Kazuo,
Kudo Toshiaki,
Horikoshi Koki
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02516.x
Subject(s) - gene , ecori , cellulase , genetics , biology , gene duplication , dna , homologous chromosome , circular bacterial chromosome , chromosome , gene cluster , microbiology and biotechnology , plasmid , base pair , enzyme , biochemistry
A 5.7‐kb Eco RI DNA fragment has been isolated from Bacillus sp. strain N‐4 chromosome DNA. This fragment contained both the pNK1‐encoded cellulase ( celB ) gene and the pNK2‐encoded cellulase ( celA ) gene which were highly homologous [13]. These results demonstrate the tandem location of these genes on the chromosomal DNA. The homologous sequence, which may play an important role for the gene duplication, were observed 5′ upstream of the celA gene, between the celA and celB genes, and 3′ downstream from the celB gene.