Open AccessA method for harvesting non‐cultivable filamentous segmented microbes inhabiting the ileum of mice
Author(s) -
Tannock G.W.,
Crichton C.M.,
Savage D.C.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02409.x
Subject(s) - segmented filamentous bacteria , biology , ileum , microbiology and biotechnology , epithelium , biochemistry , sewage treatment , activated sludge , genetics , engineering , waste management
A method by which non‐cultivable filamentous segmented microbes colonising the ileal epithelium of mice can be harvested was devised. Intact epithelial cells with attached filamentous microbes were obtained. An ethanol‐treated preparation of epithelial material was used to restore the filamentous segmented microbes to the normal microflora of mice maintained by gnotobiotic methodology.