Open AccessThiosulfate reductase as a chlorate reductase in Salmonella typhimurium
Author(s) -
Riggs Daniel L.,
Tang Jane S.,
Barrett Ericka L.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02326.x
Subject(s) - chlorate , thiosulfate , nitrate reductase , biochemistry , chemistry , reductase , mutant , enzyme , inorganic chemistry , organic chemistry , sulfur , gene
The contribution of thiosulfate reductase to chlorate sensitivity in Salmonella typhimurium was examined. Electrophoresed extracts of nitrate‐grown cells of both the wild type and a chlC mutant were shown to contain chlorate reductase activity of the same relative mobility as a thiosulfate reductase activity which was present in the chlC mutant, but not in the wild‐type grown under these conditions. A mutation is phs , which is essential for thiosulfate reductase by S. typhimurium , was shown to confer some chlorate resistance in the wild‐type background and to increase the chlorate resistance obtained with a chlC mutation. Finally, thiosulfate in the anaerobic growth medium was shown to protect a chlC mutant growing in the presence of chlorate, but it did not protect the wild type. The results are consistent with a picture in which thiosulfate reductase can function as a chlorate reductase in both the wild‐type and chlC backgrounds, although its capacity to reduce thiosulfate is diminished by the presence of an active nitrate reductase encoded by chlC .