Open AccessAntiserum against Xanthomonas phytopathogen inhibits host—Pathogen interaction in seedlings of Brassica campestris
Author(s) -
Roberts Ian N.,
Dow J.Maxwell,
Lum Keng Yeang,
Scofield Graham,
Barber Christine E.,
Daniels Michael J.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02318.x
Subject(s) - antiserum , xanthomonas campestris , polyclonal antibodies , xanthomonas campestris pv. campestris , pathogen , biology , microbiology and biotechnology , crucifer , xanthomonas , pseudomonadaceae , black rot , bacteria , antigen , botany , pseudomonas , genetics
Xanthomonas campestris pv campestris ( X.c.c. ), the causal agent of crucifer black rot, causes turnip seedlings to collapse and rot. X.c. pv vitians ( X.c.v. ) is a non‐pathogen of turnips and triggers a resistance response. Transconjugants of X.c.c. , containing defined fragments of DNA from X.c.v. , which have reduced pathogenicity to turnips were constructed. A polyclonal antiserum raised against X.c.v. restored the pathogenicity of one of these transconjugants. Antiserum ‘purged’ by reaction with sonicates of X.c.c. was equally effective in restoring pathogenicity. The purged antiserum recognised considerably fewer components than the original antiserum following Western blotting of bi‐dimensional gels. This subset of antigens appeared to be localised primarily on the bacterial cell surface. The results indicate the potential use of purged polyclonal antisera in identifying bacterial determinants that trigger host resistance responses.