Open AccessRegulation of manX ( ptsL ) and manY ( pel ) genes required for mannose transport and penetration of λDNA in Escherichia coli K12
Author(s) -
Saris Per E.J.,
Palva E.Tapio
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02317.x
Subject(s) - biology , gene , escherichia coli , fusion gene , microbiology and biotechnology , lac operon , operon , molecular cloning , locus (genetics) , cloning (programming) , genetics , gene expression , computer science , programming language
The manXYZ ( ptsL, pel/ptsM ) locus consists of 3 genes involved in mannose transport in Escherichia coli K12. In addition, the manY gene product is required for penetration of λDNA into the cell. We have previously constructed a series of lacZ gene fusions to manYZ . In this paper we describe the molecular cloning and regulatory properties of these fusions. A λ transducing phage was isolated that carried both the manX gene and the manYZ‐lacZ gene fusion. The manX gene together with the fusion was subcloned into the fusion cloning vector pMLB524. Analysis of these subclones showed that lacZ was fused to manY . The manX and manY genes appeared to be in the same operon, manX being the promoter‐proximal gene.