Open AccessThe ptsL, pel/ptsM ( manXYZ ) locus consists of three genes involved in mannose uptake in Escherichia coli K12
Author(s) -
Saris Per E.J.,
Palva E.Tapio
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02316.x
Subject(s) - subcloning , locus (genetics) , complementation , mannose , biology , gene , phosphotransferase , phosphoenolpyruvate carboxykinase , genetics , pep group translocation , microbiology and biotechnology , escherichia coli , biochemistry , mutant
The pel/ptsM locus codes for two apparently unrelated activities: transport of mannose by the phosphoenolpyruvate‐dependent phosphotransferase system (PTS) and penetration of bacteriophage λ‐DNA. This locus is known to consist of at least two genes, ptsL ( manX ) and pel/ptsM ( manYZ ). In this paper we present the cloning and analysis of the manXYZ locus. Subcloning and complementation analysis of the different fragments suggest that the locus contains 3 separate genes manX, manY and manZ and that the corresponding gene products are involved in mannose uptake. In addition, manY codes for the Pel function, necessary for penetration of λ‐DNA.