Open AccessCarbohydrate specific binding of fibronectin to Vibrio cholerae cells
Author(s) -
Wiersma Erik J.,
Fröman Gunnar,
Johansson Staffan,
Wadström Torkel
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02315.x
Subject(s) - fibronectin , vibrio cholerae , glycoprotein , biochemistry , chemistry , microbiology and biotechnology , fibronectins , biology , cell , bacteria , genetics
Cells of 10 strains of Vibrio cholerae were grown on Trypticase Soy Broth and were tested for different surface porperties such as expression of surface haemagglutinins, cell‐surface hydrophobicity and binding to 3 connective tissue proteins: fibronectin, type II collagen and fibrinogen. All strains bound fibronectin and one selected strain was shown to bind in a time‐dependent and saturable manner. The binding of 125 I‐labelled fibronectin could be completely inhibited by unlabelled fibronectin, and also partly by some other glycoproteins. Mannose inhibited binding of fibronectin up to 60%. The data indicate that carbohydrate structures within the 40 kDa (gelatin binding) and 105 kDa (cell binding) fragments of fibronectin are recognized by lectins on V. cholerae . The binding of collagen or fibrinogen was low or negligible.