Open AccessDeoxyribonucleases of non‐pathogenic corynebacteria
Author(s) -
Rama J.M.,
Sandoval H.,
Pons M.E.,
Lopez P.,
Martin J.F.,
Espinosa M.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02312.x
Subject(s) - deoxyribonucleases , deoxyribonuclease , dna , chemistry , sodium dodecyl sulfate , microbiology and biotechnology , proteases , agar , brevibacterium , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , corynebacterium , bacteria , enzyme , biology , microorganism , genetics
Fourteen species of non‐pathogenic corynebacteria have been examined for the presence of DNases. Seven of the species were found to be DNase positive when assayed in Toluidine Blue‐DNA‐containing agar whereas only one, Corynebacterium callunae , could be detectable as DNase positive when the test was performed in DNA‐methyl green‐containing agar. Electrophoretic patterns obtained from sodium dodecyl sulfate‐polyacrylamide gels containing DNA showed the presence of one or two bands of nucleolytic activity in two species of Brevibacterium and of several bands in C. callunae . Degradation of DNases by cellular proteases seem to occur in this species.