Open AccessIntergeneric complementation by Agrobacterium tumefaciens chromosomal genes and its potential use for linkage mapping
Author(s) -
Waelkens Franciska,
Verdickt Kathelijne,
Vanduffel Lia,
Vanderleyden Jos,
Gool August,
Mergeay Max
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02310.x
Subject(s) - agrobacterium tumefaciens , complementation , biology , genetics , plasmid , auxotrophy , ti plasmid , agrobacterium , transformation (genetics) , kanamycin , pseudomonas fluorescens , strain (injury) , chromosome , gene , escherichia coli , bacteria , phenotype , anatomy
The IncP1 type plasmids pULB113 (RP4:: mini‐Mu) and pJB3JI were used for chromosome mobilization and R prime formation in Agrobacterium tumefaciens . With pULB113, R primes were selected for complementation of an auxotrophic marker in an Alcaligenes eutrophus recipient strain. With pJB3JI, R primes containing the Agrobacterium chromosomal virulence region chv , inactivated by a Tn 5 insertion, were constructed. Selection was for kanamycin resistance in E. coli strain GV1000. Complementation tests were performed in auxotrophic A. tumefaciens, A. eutrophus and Pseudomonas fluorescens strains. This allowed us to map 9 loci, including the chv loci, in a region accounting for about 10% of the Agrobacterium chromosome.