Open AccessEnhanced invasion by enteroinvasive Escherichia coli of multinucleated HeLa cells
Author(s) -
Bessen Debra,
Gotschlich Emil C.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02307.x
Subject(s) - hela , multinucleate , cell , escherichia coli , cell fusion , giant cell , polyethylene glycol , biology , peg ratio , cell culture , chemistry , microbiology and biotechnology , biochemistry , genetics , finance , economics , gene
Enteroinvasive Escherichia coli (EIEC) preferentially invaded multinucleated HeLa cells which arose naturally in monolayer cultures at a low frequency. Data suggests that enhancement of the penetration step contributes to increased invasion. Treatment of HeLa cells with polyethylene glycol (PEG) led to cell fusion, and invasion of PEG‐treated monolayers by EIEC increased an average of 7‐fold compared to untreated HeLa cells. Large, multinucleated cells generated by PEG showed a striking accumulation of EIEC in cellular extensions. This study demonstrates that alteration of HeLa cell morphology results in increased invasion by EIEC.