Open AccessCloning of the gene coding for staphylococcal exfoliative toxin A and its expression in Escherichia coli
Author(s) -
Sakurai Susumu,
Suzuki Hitoshi,
Kondo Isamu
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02300.x
Subject(s) - escherichia coli , plasmid , microbiology and biotechnology , biology , recombinant dna , periplasmic space , gene , molecular cloning , cloning (programming) , toxin , staphylococcus aureus , gene expression , genetics , bacteria , computer science , programming language
The gene for the staphylococcal exfoliative toxin of serotype A (ETA) was cloned from the genomic DNA into pUC9 and expressed in Escherichia coli C600. Recombinant plasmid pETA‐J3 was recovered from an ETA‐producing transformant and the eta gene was shown to be located within a 1.7‐kb Alu I fragment. ETA produced by the E. coli transformant, cETA, was indistinguishable from that produced by Staphylococcus aureus, sETA, in its physicochemical and serological properties and exfoliative toxicity. cETA was not retained in the periplasmic space; and more than 97% of the toxin was extracellular.