Open AccessReactivity of elementary and reticulate bodies of Chlamydia trachomatis LGV2 with monoclonal antibodies specific for the major outer membrane protein
Author(s) -
Cevenini R.,
Donati M.,
Sambri V.,
Rumpianesi F.,
La Placa M.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02297.x
Subject(s) - monoclonal antibody , chlamydia trachomatis , immunofluorescence , biology , antibody , reticulate , chlamydiales , bacterial outer membrane , serotype , chlamydiaceae , microbiology and biotechnology , monoclonal , virology , escherichia coli , immunology , biochemistry , gene , botany
The reactivity of the major outer membrane protein (MOMP) of Chlamydia trachomatis (LGV2 serotype) with 15 monoclonal antibodies was studied during the course of developmental cycle by immunoblotting and immunofluorescence. The monoclonal antibodies reacted in immunoblots with the MOMP of both elementary bodies (EBs) and reticulate bodies (RBs). Using an immunofluorescence test with LGV2‐infected cell cultures, the 15 monoclonal antibodies could be divided into 5 groups, according to the time of appearance of their reactivity with the cell culture.