Open AccessTn 5 insertion in kdgR , a regulatory gene of the polygalacturonate pathway in Erwinia chrysanthemi
Author(s) -
Condemine Guy,
RobertBaudouy Janine
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02296.x
Subject(s) - mutant , transposable element , biology , genetics , gene , mutagenesis , regulator gene , insertion , locus (genetics) , transposon mutagenesis , microbiology and biotechnology , regulation of gene expression
Tn 5 mutagenesis was performed on the Erwinia chrysanthemi strain 3937 with the transposon vector pMO194. The vector is spontaneously lost from the cell and Tn 5 transposes randomly on the chromosome. A mutant with a derepressed expression of a kduD‐lac fusion was obtained. In this mutant, Tn 5 was inserted in a regulatory gene, named kdgR, resulting in constitutive expression of genes of the polygalacturonate degradative pathway ( pelA, pelD, pelE, ogl, kduI, kduD, kdgT, kdgK and kdgA ). Constitutive expression of these genes after inactivation of the kdgR gene by Tn 5 insertion suggests that kdgR is a negative regulatory gene. The kdgR mutation was mapped near the trp locus on the E. chrysanthemi chromosome.