Open AccessThe Pasteur effect in yeasts: Mass spectrometric monitoring of oxygen uptake, and carbon dioxide and ethanol production
Author(s) -
Lloyd D.,
James C.J.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02294.x
Subject(s) - ethanol , chemistry , respiratory quotient , carbon dioxide , respiration , oxygen , biochemistry , catabolite repression , fermentation , glycolysis , saccharomyces cerevisiae , yeast , anaerobic exercise , ethanol fuel , nuclear chemistry , food science , chromatography , metabolism , organic chemistry , biology , botany , physiology , gene , mutant
Simultaneous and continuous monitoring of dissolved O 2 , CO 2 and ethanol in suspensions of Saccharomyces uvarum and Saccharomyces cerevisiae , by means of a quadrupole mass spectrometer fitted with a silicone rubber membrane inlet, enabled a study of O 2 inhibition of glycolysis (Pasteur effect). Calculation of Pasteur quotients (ratios of anaerobic to aerobic glycolytic rates) give similar values when corrected both for respiratory CO 2 production and ethanol oxidation under aerobic conditions. For S. cerevisiae the apparent K m for O 2 of the respiration of catabolite derepressed cells was 1 μM and the apparent K i values for O 2 as an inhibitor of glycolysis were 12.7 and 7 μM, respectively, when measurements were made on CO 2 evolution and ethanol production.