Open AccessProduction of colominic acid by Pasteurella haemolytica serotype A2 organisms
Author(s) -
Adlam C.,
Knights J.M.,
Mugridge Anne,
Williams Janet M.,
Lindon J.C.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02293.x
Subject(s) - immunogenicity , microbiology and biotechnology , polysaccharide , neisseria meningitidis , serotype , bacterial capsule , strain (injury) , escherichia coli , pathogen , bacteria , chemistry , nuclear magnetic resonance spectroscopy , biology , virulence , biochemistry , stereochemistry , antigen , gene , immunology , genetics , anatomy
Using chemical analysis and 13 C‐nuclear magnetic resonance (NMR) spectroscopy, capsular polysaccharide purified from culture supernatants of a strain of Pasteurella haemolytica serotype A2 was shown to consist of a (2 → 8)‐α‐linked polymer of N ‐acetylneuraminic acid. This is identical to the capsular polysaccharides of Neisseria meningitidis group B and Escherichia coli K1, and is known as colominic acid. Polymer isolated from a second strain was contaminated with α‐1,4‐linked dextran. The known poor immunogenicity of these two polymers explains the failure by others to produce effective extract vaccines for this important ovine pathogen.