Open AccessDetermination of 3‐deoxy‐ d ‐manno‐2‐octulosonic acid (KDO) in Legionella pneumophila by gas chromatography
Author(s) -
Guerrant Gordon O.,
Wong K.H.,
Moss C.Wayne
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02289.x
Subject(s) - legionella pneumophila , chromatography , hydrolysate , lipopolysaccharide , chemistry , gas chromatography , hydrolysis , gas chromatography–mass spectrometry , legionella , acid hydrolysis , mass spectrometry , biochemistry , bacteria , biology , genetics , endocrinology
A structural component of lipopolysaccharide, 3‐deoxy‐ d ‐manno‐2‐octulosonic acid (KDO), was determined in Legionella pneumophila by gas‐liquid chromatography (GLC) and confirmed by gas chromatography/mass spectrometry (GC/MS), using isolated lipopolysaccharide (LPS) and whole cells of L. pneumophila . KDO was liberated from cellular components by acid hydrolysis, and the dried hydrolysate was derivatized to form volatile and stable compounds for analysis by GLC. The GLC procedure overcomes some deficiencies of the conventional colorimetric thiobarbituric acid (TBA) method, and has adequate resolution to determine KDO in hydrolyzed whole cells without interference from other cellular components. The KDO content of the LPS was determined to be 0.53% which is in agreement with 0.65% previously found by the TBA method.