Open AccessBiosynthesis of monensins and 3‐ O ‐demethylmonensins in Streptomyces cinnamonensis in the presence of methylation inhibitors
Author(s) -
Pospišil Stanislav,
Zima Jiři
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02283.x
Subject(s) - streptomyces , methylation , biosynthesis , streptomycetaceae , actinomycetales , biochemistry , biology , chemistry , stereochemistry , genetics , bacteria , gene
We studied the effect of inhibitors of methylation ( p ‐aminosalicylic acid, sulfonamides, aminopterine) on the biosynthesis of the oligoketide antibiotics, monensins A and B, and their cometabolites, 3‐ O ‐demethylmonensins A and B. The inhibitors were supplied to the culture medium of Streptomyces cinnamonensis LO‐63 at the beginning or in the 17th hour of cultivation. Under noninhibitory conditions 3‐ O ‐demethylmonensins represent 2% total monensins at the most. The highest relative yield of demethylated monensins was achieved with aminopterine (2.3 · 10 −2 mM) added in the 17th hour. A high proportion of 3‐ O ‐demethylated monensins, 28–30% of the total production, was brought about by sulfadimidine and sulfathiazole (20 mM); however, the agents also caused a substantial inhibition of the overall production. Sulfaguanidine and phthalylsulfathiazole were poor inhibitors of methylation, sulfisoxazole and p ‐aminosalicylic acid had no effect. The strong effect of aminopterine on the biosynthesis of 3‐ O ‐demethylmonensins was confirmed in the regulatory mutant S. cinnamonensis ACB‐ABR‐2 which produces mainly monensin A.