Open AccessAnalysis of antigenic reactivity of synthetic monosaccharide lipid A analogues with monoclonal antibodies
Author(s) -
Arata S.,
Mashimo J.,
Kasai N.,
Okuda K.,
Aihara Y.,
Hasegawa A.,
Kiso M.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02273.x
Subject(s) - monoclonal antibody , monosaccharide , antigen , chemistry , antibody , epitope , reactivity (psychology) , biochemistry , biology , immunology , medicine , alternative medicine , pathology
In vitro antigenic reactivity of chemically synthesized monosaccharide analogues of nonreducing sugar moiety of lipid A with 4 monoclonal antibodies against Salmonella minnesota R595 lipid A was studied by the inhibition test of enzyme‐linked immunosorbent assay (ELISA). In the assays with 2 monoclonal antibodies (mAb 5G and mAb36G), which have been suggested to recognize the Salmonella type lipid A structure, no antigenic reactivity of the tested analogues was observed. However, several monosaccharide analogues exhibited antigenic reactivities with other monoclonal antibodies (mAb161M and mAb19M), especially some structural differences of hydrophobic and/or hydrophilic parts among monosaccharide analogues were recognized by mAb161M.