Open AccessPreliminary characterization of Chlamydia trachomatis hemagglutinins
Author(s) -
Chong Karen L.,
Kaul Ravi,
Sherburne Richard,
Wenman Wanda M.
Publication year - 1987
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1987.tb02272.x
Subject(s) - chlamydia trachomatis , hemagglutination , hemagglutinin (influenza) , antiserum , biology , chlamydiaceae , microbiology and biotechnology , serotype , antigen , membrane , biochemistry , virology , immunology
The hemagglutination activity of Chlamydia trachomatis serovar L 2 whole elementary bodies (EB) was compared to EB membranes prepared by deoxycholate treatment and to infected HeLa cells solubilized in octylglucoside. Differential responses of these extracts to various erythrocytes, chlamydial antisera, temperature and heating indicated that two hemagglutinins are present in the outer membrane of C. trachomatis . The hemagglutinin associated with whole EB and released by octylglucoside is heat‐sensitive and proteinaceous, while that associated with EB membranes is heat‐resistant and non‐antigenic. Our data suggest that the latter hemagglutinin is non‐proteinaceous in nature and is common to both chlamydial life forms, EB and reticulate bodies (RB). The affinity of 3 H‐labeled EB for different red blood cell species closely paralleled the degree of hemagglutination observed.